T Cell Assay

Human T cell assays

T lymphocytes

T cells are primarily responsible for mediating cellular immunity. They originate in the bone marrow and undergo maturation in the thymus. Within the thymus, T cells undergo proliferation and differentiation into helper, regulatory, cytotoxic T cells, or memory T cells. Subsequently, they are dispatched to peripheral tissues or circulate within the blood or lymphatic system. Upon stimulation by specific antigens, helper T cells secrete cytokines that facilitate B cell differentiation. Regulatory T cells function to modulate immune responses while cytotoxic T cells, activated by diverse cytokines, bind to and eliminate infected as well as cancerous target cells.

Our services include:

T cell activation and proliferation (MLR, anti-CD3/CD28; PHA/Con A, PMA/ionomycin, SEB, anti-PD1, anti-CTLA4) via high throughput Flow Cytometry and other methods (such as CFSE dilution, BrdU incorporation, and DELFIA)
Antigen-specific recall response assay
T-cell Dependent Cellular Cytotoxicity (TDCC) assay for characterization of bispecific T cell engager antibodies (BiTE)
Target expression profiling on cell subsets (resting and activated)
Screening for the ability to reverse T cell exhaustion
CD8+ T cell suppression assay with polarized Treg cells or cytokine-derived CD33+ Myeloid-Derived Suppressor Cells (MDSC)
Treg Suppression Assay
iTreg Differentiation Assay
Modulation of differentiation and cytokine profile (from CD4+ to Th1,Th2,Th17, Treg cells)

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T cell activation

T cell migration and chemotaxis transwell assay
Gamma delta (γδ) T cell cytotoxicity assay
Mixed lymphocyte reaction (PBMC/T cell/DC, one way or two-way)
Immune checkpoint blockade assay
Cytokine secretion profile (ELISA, Luminex, AlphaLISA, Lumit, or ELISpot)
T Cell Surface marker expression analysis (flow cytometry)
IL-2 Stimulation Assay (phospho-Flow, pSTAT5, etc)
T Cell-Mediated Exhaustion Assay
T Cell-Mediated Chemotaxis Assay
Tumor Killing Assays (TKA)
On/off Target Testing for TCR Cellular Therapeutics
TAA-specific T Cell Response Assays
Immune Synapse Formation Assay
3D Spheroid T Cell-Mediated Cytotoxicity Assay

Mixed Lymphocyte Reaction (MLR) Assay

About MLR Assay

Our Service features

The MLR assay is a platform for evaluating compounds that modulate the interaction between antigen-presenting cells (APCs) and T cells, thereby activating, deactivating, or repolarizing lymphocyte cell responses. This assay enables the assessment of immunomodulatory agents' efficacy and provides crucial information on drug safety regarding immunological responsiveness.
Our MLR Assay facilitates the rapid identification of T cell-modulating agents and is commonly employed to evaluate biologics or small molecules as single agents or in combination in vitro.
Leveraging our extensive expertise in ultrasensitive and multiplex immunoassays, flow cytometric analysis, as well as knowledge in immunology and oncology, we offer reliable analysis and interpretation of test agent effects on multiple endpoints using various allogeneic donor pairs (e.g., human PBMC/CD3+/CD4+ T/DC cells, mouse splenocytes, etc.).

One-way MLR is an assay for lymphocyte proliferation, in which responder lymphocytes (such as CD4+ T cells) from one donor are stimulated to proliferate by stimulator lymphocytes from another donor. The stimulator cells (such as allogeneic DCs) are pre-treated with mitomycin-c. Lymphocytes are co-cultured for several days, and the quantification of secreted cytokines (such as IL-2, IFN-g) in cell culture is performed. Bromo-deoxyuridine (BrdU) is introduced into the cell culture to determine the presence of proliferating T cells.
A two-way MLR differs from a one-way MLR in that both donors of lymphocytes stimulate each other and have the ability to proliferate.

Our Service features

High throughput: 96~384-well assay
Robust and highly reproducible assay suitable for small to large scale screening
Various applications: Applicable for drug safety, immuno-oncology, and autoimmunity, etc
Single agents or combinations: Flexible assay design to fit specific project
Multiple allogeneic donors: PBMC or enriched primary T cells with various genotypes (such as FcγRIIIa 158 V/V) and monocytes derived dendritic cells (Mo-DCs) from multiple donors to address donor-to-donor variability
Multiple endpoints: Surface markers, cytokines (such as IL-2, IFN-r), and cell proliferation clearly understand the immuno-modulatory profile of test agents
State-of-art platforms: Well calibrated and verified Luminex-200 or FlexMAP 3D for multiplex cytokine analysis; CytoFLEX S (Flow cytometer with 4 lasers 13 colors) for cell identification and quantification.
Quick turn-around time: concise and robust quantitative data in as little as 4 weeks
Fully validated and quality-controlled
Multiple concentrations in triplicate
Positive and negative controls: isotype Ig G, inhibitor and stimulator controls
Over 20+ years of hands-on experience: Expert data analysis and interpretation, scientific and technical support
Quality publication-style study report

T Cell Functional Assays

Human T cell assays

T lymphocytes

T cell activation

Mixed Lymphocyte Reaction (MLR) Assay

About MLR Assay

Our Service features

Our Service features

Our Service features

Our Service features

Our Service features

T cell activity assay data examples

T Cell Functional Assays

Human T cell assays

T lymphocytes

T cell activation

Mixed Lymphocyte Reaction (MLR) Assay

About MLR Assay

Our Service features

Our Service features

Our Service features

Our Service features

Our Service features

T cell activity assay data examples

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